visium spatial gene expression reagent v2 kit  (10X Genomics)

Journal: Cell Reports Medicine

Article Title: A longitudinal, multi-omic atlas reveals the emergence of a spatially organized immunosuppressive ecosystem in resistant melanoma

doi: 10.1016/j.xcrm.2026.102716

Figure Lengend Snippet: Overview of longitudinal tumor biopsies and multi-platform analysis (A) Longitudinal tumor specimens ( n = 45) from 10 patients with metastatic melanoma. (B and C) Treatment timelines and clinical events for the MGH patient 35 (Pt35) (B) and the MGH patient 42 (Pt42) (C). Pt35 is an R, and Pt42 is an NR to anti-PD-1 therapy. (D) Bulk tumor profiling workflow incorporating WES, RNA-seq, NanoString Vantage 3D, and RPPA. (E) Spatial analysis utilizing NanoString DSP, CyCIF, mIHC, and Visium HD. See also and .

Article Snippet: Visium HD Spatial Gene Expression Reagent Kit , 10x Genomics , PN-1000675.

Techniques: RNA Sequencing

Journal: bioRxiv

Article Title: OmicGlaze: Spatial Multi-Omic Mapping of Traumatic Brain Injury

doi: 10.64898/2026.03.30.715462

Figure Lengend Snippet: A A focal blunt injury was induced in the upper right hemisphere of the mouse brain. Serial coronal sections were obtained at the mid-hippocampal level, with adjacent sections allocated for histological analysis (hematoxylin and eosin staining and Nissl staining), spatial RNA sequencing (spatial RNA-seq), and spatial ATAC sequencing (spatial ATAC-seq). B Image of a Nissl-stained section adjacent to the TBI_03 sample used for sequencing, illustrating tissue morphology. The left side corresponds to the uninjured hemisphere, and the right side to the injured hemisphere. C Zoomed-in view of the isocortex on the uninjured side of panel B. Scale bar represents 250 µm. D Zoomed-in view of the isocortex on the injured side of panel B. Scale bar represents 250 µm. E Spatial distribution of the number of detected genes per spot in the Control_01 sample. Each dot represents a 55-micron spot on the Visium slide (11 x 11 mm 2 ). F - H Spatial distribution of the number of detected genes per spot in the TBI_01, TBI_02, and TBI_03 samples. I Spatial region annotation of the TBI_03 sample, highlighting 14 anatomical regions. Abbreviations: CNU, cerebral nuclei; CTX_ISO, isocortex; CTX_OLF, olfactory cortex; CTX_RSP, retrosplenial area; CTX_SP, cortical subplate; HPF_CA_PYR, hippocampal formation cornu ammonis pyramidal layer; HPF_DG_GCL, hippocampal formation dentate gyrus granule cell layer; HPF_other, the polymorphic layer and molecular layer of hippocampus; HY, hypothalamus; LM, leptomeninges; TH, thalamus; VS, ventricular systems. J Unsupervised clustering of the integrated dataset comprising Control_01, TBI_01, TBI_02, and TBI_03 samples, colored by brain regions as defined in panel I. K Heatmap showing the expression levels of the top five marker genes for each of the 14 annotated regions in Control_01. Each row represents one gene and each column under the same color represents one region. Selected marker genes are labeled on the plot: Adora2a (marker for CNU region), Apod (fiber tracts), Satb2 (CTX_ISO region), Dlk1 (HY region), Synop2 (TH region), Hopx ( HPF_other region), Nrep (CTX_RSP region), Ttr (VS region), and Gpr151 (habenula region).

Article Snippet: We used Visium V2 CytAssist Spatial Gene Expression Reagent Kits (10X Genomics, #1000523) to profile mRNAs from tissue slices.

Techniques: Staining, RNA Sequencing, Sequencing, Control, Olfactory, Expressing, Marker, Labeling