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visium spatial gene expression reagent v2 kit  (10X Genomics)

 
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    Structured Review

    10X Genomics visium spatial gene expression reagent v2 kit
    Visium Spatial Gene Expression Reagent V2 Kit, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/visium+spatial+gene+expression+reagent+kits/pm42265746-64-20-27
    Average 86 stars, based on 1 article reviews
    visium spatial gene expression reagent v2 kit - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Generated:

    Article Title: Tlr7 drives sex differences in age- and Alzheimer’s disease–related demyelination
    Article Snippet: .. Libraries were generated using Visium Spatial Gene Expression Reagent Kits (10x Genomics, PN-1000184) with dual index primers (10x Genomics, PN-1000215) and sequenced on a NovaSeq6000 using a S4 100 cycle flow cell. ..

    Gene Expression:

    Article Title: Tlr7 drives sex differences in age- and Alzheimer’s disease–related demyelination
    Article Snippet: .. Libraries were generated using Visium Spatial Gene Expression Reagent Kits (10x Genomics, PN-1000184) with dual index primers (10x Genomics, PN-1000215) and sequenced on a NovaSeq6000 using a S4 100 cycle flow cell. ..

    Article Title: Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development.
    Article Snippet: .. Following permeabilization of the tissues, cDNA synthesis, amplification, and sequencing library preparation were conducted using Visium Spatial Gene Expression Reagent Kits (10x Genomics). .. NovaSeq 6000 (Illumina) was used for sequencing.

    Article Title: Remodeling lesions locate at sites of strong extravillous trophoblast invasion and are associated with neutrophil presence in the human first-trimester decidua.
    Article Snippet: .. Tissue optimization for spatial analysis was performed according to the Visium Spatial Gene Expression Reagent Kits—Tissue Optimization User Guide (CG000238, 10x Genomics, Netherlands). .. Spatial transcriptomics Decidua basalis and parietalis tissues were dissected into cubes with �4 mm side length (n = 2 vs 2, tissues from two donors of our donor cohort).

    Article Title: Cutaneous T cell lymphoma atlas reveals malignant T H 2 cells supported by a B cell-rich tumor microenvironment.
    Article Snippet: .. All tissues were sectioned at a thickness of 15 μm and a tissue optimization experiment using Visium Spatial Gene Expression Reagent Kits—Tissue Optimization (10x Genomics) was carried out to determine the optimum permeabilization time (14 min) using the manufacturer’s protocol. .. Spatial gene expression libraries were then generated using the Visium Spatial Gene Expression Reagent Kits (10x Genomics) and the manufacturer’s protocol and sequenced using an Illumina NovaSeq 6000 to achieve a minimum number of 50,000 read pairs per tissue-covered spot.

    Article Title: Granzyme K + CD8 + T cells interact with fibroblasts to promote neutrophilic inflammation in nasal polyps.
    Article Snippet: .. Thereafter, Visium Spatial Gene Expression was conducted with Visium Spatial Gene-Expression Reagent Kits (Rev B, 10x Genomics). ..

    Article Title: Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development
    Article Snippet: .. Following permeabilization of the tissues, cDNA synthesis, amplification, and sequencing library preparation were conducted using Visium Spatial Gene Expression Reagent Kits (10x Genomics). .. NovaSeq 6000 (Illumina) was used for sequencing.

    cDNA Synthesis:

    Article Title: Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development.
    Article Snippet: .. Following permeabilization of the tissues, cDNA synthesis, amplification, and sequencing library preparation were conducted using Visium Spatial Gene Expression Reagent Kits (10x Genomics). .. NovaSeq 6000 (Illumina) was used for sequencing.

    Article Title: Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development
    Article Snippet: .. Following permeabilization of the tissues, cDNA synthesis, amplification, and sequencing library preparation were conducted using Visium Spatial Gene Expression Reagent Kits (10x Genomics). .. NovaSeq 6000 (Illumina) was used for sequencing.

    Amplification:

    Article Title: Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development.
    Article Snippet: .. Following permeabilization of the tissues, cDNA synthesis, amplification, and sequencing library preparation were conducted using Visium Spatial Gene Expression Reagent Kits (10x Genomics). .. NovaSeq 6000 (Illumina) was used for sequencing.

    Article Title: Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development
    Article Snippet: .. Following permeabilization of the tissues, cDNA synthesis, amplification, and sequencing library preparation were conducted using Visium Spatial Gene Expression Reagent Kits (10x Genomics). .. NovaSeq 6000 (Illumina) was used for sequencing.

    Sequencing:

    Article Title: Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development.
    Article Snippet: .. Following permeabilization of the tissues, cDNA synthesis, amplification, and sequencing library preparation were conducted using Visium Spatial Gene Expression Reagent Kits (10x Genomics). .. NovaSeq 6000 (Illumina) was used for sequencing.

    Article Title: Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development
    Article Snippet: .. Following permeabilization of the tissues, cDNA synthesis, amplification, and sequencing library preparation were conducted using Visium Spatial Gene Expression Reagent Kits (10x Genomics). .. NovaSeq 6000 (Illumina) was used for sequencing.

    other:

    Article Title: Cold and hot fibrosis define clinically distinct cardiac pathologies.
    Article Snippet: The slides were imaged with Axio Imager.Z2 (upright) with Zeiss Axiocam 506 Color camera.

    Article Title: Cold and hot fibrosis define clinically distinct cardiac pathologies
    Article Snippet: After all sections were placed on the Visium slide, the downstream pipeline was continued in accordance with the manufacturer's guidelines (10x genomics, Visium Spatial Gene Expression Reagent Kits User Guide).



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    Overview of longitudinal tumor biopsies and multi-platform analysis (A) Longitudinal tumor specimens ( n = 45) from 10 patients with metastatic melanoma. (B and C) Treatment timelines and clinical events for the MGH patient 35 (Pt35) (B) and the MGH patient 42 (Pt42) (C). Pt35 is an R, and Pt42 is an NR to anti-PD-1 therapy. (D) Bulk tumor profiling workflow incorporating WES, RNA-seq, NanoString Vantage 3D, and RPPA. (E) Spatial analysis utilizing NanoString DSP, CyCIF, mIHC, and <t>Visium</t> <t>HD.</t> See also and .
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    A A focal blunt injury was induced in the upper right hemisphere of the mouse brain. Serial coronal sections were obtained at the mid-hippocampal level, with adjacent sections allocated for histological analysis (hematoxylin and eosin staining and Nissl staining), spatial RNA sequencing (spatial RNA-seq), and spatial ATAC sequencing (spatial ATAC-seq). B Image of a Nissl-stained section adjacent to the TBI_03 sample used for sequencing, illustrating tissue morphology. The left side corresponds to the uninjured hemisphere, and the right side to the injured hemisphere. C Zoomed-in view of the isocortex on the uninjured side of panel B. Scale bar represents 250 µm. D Zoomed-in view of the isocortex on the injured side of panel B. Scale bar represents 250 µm. E Spatial distribution of the number of detected genes per spot in the Control_01 sample. Each dot represents a 55-micron spot on the <t>Visium</t> slide (11 x 11 mm 2 ). F - H Spatial distribution of the number of detected genes per spot in the TBI_01, TBI_02, and TBI_03 samples. I Spatial region annotation of the TBI_03 sample, highlighting 14 anatomical regions. Abbreviations: CNU, cerebral nuclei; CTX_ISO, isocortex; CTX_OLF, olfactory cortex; CTX_RSP, retrosplenial area; CTX_SP, cortical subplate; HPF_CA_PYR, hippocampal formation cornu ammonis pyramidal layer; HPF_DG_GCL, hippocampal formation dentate gyrus granule cell layer; HPF_other, the polymorphic layer and molecular layer of hippocampus; HY, hypothalamus; LM, leptomeninges; TH, thalamus; VS, ventricular systems. J Unsupervised clustering of the integrated dataset comprising Control_01, TBI_01, TBI_02, and TBI_03 samples, colored by brain regions as defined in panel I. K Heatmap showing the expression levels of the top five marker genes for each of the 14 annotated regions in Control_01. Each row represents one gene and each column under the same color represents one region. Selected marker genes are labeled on the plot: Adora2a (marker for CNU region), Apod (fiber tracts), Satb2 (CTX_ISO region), Dlk1 (HY region), Synop2 (TH region), Hopx ( HPF_other region), Nrep (CTX_RSP region), Ttr (VS region), and Gpr151 (habenula region).
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    Image Search Results


    Overview of longitudinal tumor biopsies and multi-platform analysis (A) Longitudinal tumor specimens ( n = 45) from 10 patients with metastatic melanoma. (B and C) Treatment timelines and clinical events for the MGH patient 35 (Pt35) (B) and the MGH patient 42 (Pt42) (C). Pt35 is an R, and Pt42 is an NR to anti-PD-1 therapy. (D) Bulk tumor profiling workflow incorporating WES, RNA-seq, NanoString Vantage 3D, and RPPA. (E) Spatial analysis utilizing NanoString DSP, CyCIF, mIHC, and Visium HD. See also and .

    Journal: Cell Reports Medicine

    Article Title: A longitudinal, multi-omic atlas reveals the emergence of a spatially organized immunosuppressive ecosystem in resistant melanoma

    doi: 10.1016/j.xcrm.2026.102716

    Figure Lengend Snippet: Overview of longitudinal tumor biopsies and multi-platform analysis (A) Longitudinal tumor specimens ( n = 45) from 10 patients with metastatic melanoma. (B and C) Treatment timelines and clinical events for the MGH patient 35 (Pt35) (B) and the MGH patient 42 (Pt42) (C). Pt35 is an R, and Pt42 is an NR to anti-PD-1 therapy. (D) Bulk tumor profiling workflow incorporating WES, RNA-seq, NanoString Vantage 3D, and RPPA. (E) Spatial analysis utilizing NanoString DSP, CyCIF, mIHC, and Visium HD. See also and .

    Article Snippet: Visium HD Spatial Gene Expression Reagent Kit , 10x Genomics , PN-1000675.

    Techniques: RNA Sequencing

    A A focal blunt injury was induced in the upper right hemisphere of the mouse brain. Serial coronal sections were obtained at the mid-hippocampal level, with adjacent sections allocated for histological analysis (hematoxylin and eosin staining and Nissl staining), spatial RNA sequencing (spatial RNA-seq), and spatial ATAC sequencing (spatial ATAC-seq). B Image of a Nissl-stained section adjacent to the TBI_03 sample used for sequencing, illustrating tissue morphology. The left side corresponds to the uninjured hemisphere, and the right side to the injured hemisphere. C Zoomed-in view of the isocortex on the uninjured side of panel B. Scale bar represents 250 µm. D Zoomed-in view of the isocortex on the injured side of panel B. Scale bar represents 250 µm. E Spatial distribution of the number of detected genes per spot in the Control_01 sample. Each dot represents a 55-micron spot on the Visium slide (11 x 11 mm 2 ). F - H Spatial distribution of the number of detected genes per spot in the TBI_01, TBI_02, and TBI_03 samples. I Spatial region annotation of the TBI_03 sample, highlighting 14 anatomical regions. Abbreviations: CNU, cerebral nuclei; CTX_ISO, isocortex; CTX_OLF, olfactory cortex; CTX_RSP, retrosplenial area; CTX_SP, cortical subplate; HPF_CA_PYR, hippocampal formation cornu ammonis pyramidal layer; HPF_DG_GCL, hippocampal formation dentate gyrus granule cell layer; HPF_other, the polymorphic layer and molecular layer of hippocampus; HY, hypothalamus; LM, leptomeninges; TH, thalamus; VS, ventricular systems. J Unsupervised clustering of the integrated dataset comprising Control_01, TBI_01, TBI_02, and TBI_03 samples, colored by brain regions as defined in panel I. K Heatmap showing the expression levels of the top five marker genes for each of the 14 annotated regions in Control_01. Each row represents one gene and each column under the same color represents one region. Selected marker genes are labeled on the plot: Adora2a (marker for CNU region), Apod (fiber tracts), Satb2 (CTX_ISO region), Dlk1 (HY region), Synop2 (TH region), Hopx ( HPF_other region), Nrep (CTX_RSP region), Ttr (VS region), and Gpr151 (habenula region).

    Journal: bioRxiv

    Article Title: OmicGlaze: Spatial Multi-Omic Mapping of Traumatic Brain Injury

    doi: 10.64898/2026.03.30.715462

    Figure Lengend Snippet: A A focal blunt injury was induced in the upper right hemisphere of the mouse brain. Serial coronal sections were obtained at the mid-hippocampal level, with adjacent sections allocated for histological analysis (hematoxylin and eosin staining and Nissl staining), spatial RNA sequencing (spatial RNA-seq), and spatial ATAC sequencing (spatial ATAC-seq). B Image of a Nissl-stained section adjacent to the TBI_03 sample used for sequencing, illustrating tissue morphology. The left side corresponds to the uninjured hemisphere, and the right side to the injured hemisphere. C Zoomed-in view of the isocortex on the uninjured side of panel B. Scale bar represents 250 µm. D Zoomed-in view of the isocortex on the injured side of panel B. Scale bar represents 250 µm. E Spatial distribution of the number of detected genes per spot in the Control_01 sample. Each dot represents a 55-micron spot on the Visium slide (11 x 11 mm 2 ). F - H Spatial distribution of the number of detected genes per spot in the TBI_01, TBI_02, and TBI_03 samples. I Spatial region annotation of the TBI_03 sample, highlighting 14 anatomical regions. Abbreviations: CNU, cerebral nuclei; CTX_ISO, isocortex; CTX_OLF, olfactory cortex; CTX_RSP, retrosplenial area; CTX_SP, cortical subplate; HPF_CA_PYR, hippocampal formation cornu ammonis pyramidal layer; HPF_DG_GCL, hippocampal formation dentate gyrus granule cell layer; HPF_other, the polymorphic layer and molecular layer of hippocampus; HY, hypothalamus; LM, leptomeninges; TH, thalamus; VS, ventricular systems. J Unsupervised clustering of the integrated dataset comprising Control_01, TBI_01, TBI_02, and TBI_03 samples, colored by brain regions as defined in panel I. K Heatmap showing the expression levels of the top five marker genes for each of the 14 annotated regions in Control_01. Each row represents one gene and each column under the same color represents one region. Selected marker genes are labeled on the plot: Adora2a (marker for CNU region), Apod (fiber tracts), Satb2 (CTX_ISO region), Dlk1 (HY region), Synop2 (TH region), Hopx ( HPF_other region), Nrep (CTX_RSP region), Ttr (VS region), and Gpr151 (habenula region).

    Article Snippet: We used Visium V2 CytAssist Spatial Gene Expression Reagent Kits (10X Genomics, #1000523) to profile mRNAs from tissue slices.

    Techniques: Staining, RNA Sequencing, Sequencing, Control, Olfactory, Expressing, Marker, Labeling